Multi-channel biphasic calcium phosphate granules as cell carrier capable of supporting osteogenic priming of mesenchymal stem cells
- Authors
- Abueva, Celine D. G.; Park, Chan Mi; Kim, Boram; Lee, Byong-Taek
- Issue Date
- 5-Mar-2018
- Publisher
- Elsevier BV
- Keywords
- Multi-channel granule; Cell carrier; Scaffold; Stem cell; Bone; Regenerative medicine
- Citation
- Materials & Design, v.141, pp 142 - 149
- Pages
- 8
- Journal Title
- Materials & Design
- Volume
- 141
- Start Page
- 142
- End Page
- 149
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6107
- DOI
- 10.1016/j.matdes.2017.12.040
- ISSN
- 0264-1275
1873-4197
- Abstract
- Advances in bone tissue engineering include versatile and intricate biomaterial scaffolds in combination with stem cells for enhanced bone regeneration. In this study, a unique scaffold with multi-channels designed to allow cell infiltration within its pores was investigated for its capability to serve as a stable platform for adhesion and osteogenic priming of mesenchymal stem cells. The biphasic calcium phosphate multi-channel granule consisted of 60% hydroxyapatite and 40% beta-tricalcium phosphate. Successful loading and retention of isolated and expanded rat bone marrow-derived mesenchymal stem cells (rBMSCs) were observed. The cells proliferated within the micro-channels starting from the surface then into the channels. The multi-channel granules were also able to support osteogenic priming of rBMSCs in 2D culture without the aid of a growth factor. Alkaline phosphatase, type I collagen, and runt-related transcription factor 2 expressions were detected with high osteopontin marker expression in as early as 7 days, which persisted for 14 days of culture under osteogenic condition. Results confirmed commitment towards osteogenic lineage of rBMSCs that have attached and grown onto the surface of the multi-channel granules and thus have high potential as a cell-scaffold based approach in bone regenerative medicine. (C) 2017 Published by Elsevier Ltd.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Regenerative Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6107)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.