Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix
- Authors
- Kim, In Gul; Gil, Chang-Hyun; Seo, Joseph; Park, Soon-Jung; Subbiah, Ramesh; Jung, Taek-Hee; Kim, Jong Soo; Jeong, Young-Hoon; Chung, Hyung-Min; Lee, Jong Ho; Lee, Man Ryul; Moon, Sung-Hwan; Park, Kwideok
- Issue Date
- Jan-2018
- Publisher
- Pergamon Press Ltd.
- Keywords
- Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix
- Citation
- Biomaterials, v.150, pp 100 - 111
- Pages
- 12
- Journal Title
- Biomaterials
- Volume
- 150
- Start Page
- 100
- End Page
- 111
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/6374
- DOI
- 10.1016/j.biomaterials.2017.10.016
- ISSN
- 0142-9612
1878-5905
- Abstract
- Cell-derived matrices (CDM) are becoming an attractive alternative to conventional biological scaffolding platforms due to its unique ability to closely recapitulate a native extracellular matrix (ECM) de novo. Although cell-substrate interactions are recognized to be principal in regulating stem cell behavior, very few studies have documented the acclimation of human pluripotent stem cells (hPSCs) on pristine and altered cell-derived matrices. Here, we investigate crosslink-induced mechanotransduction of hPSCs cultivated on decellularized fibroblast-derived matrices (FDM) to explore cell, adhesion, growth, migration, and pluripotency in various biological landscapes. The results showed either substrate-mediated induction or inhibition of the Epithelial-Mesenchymal-Transition (EMT) program, strongly suggesting that FDM stiffness can be a dominant factor in mediating hPSC plasticity. We further propose an optimal FDM substratum intended for long-term hPSC cultivation in a feeder-free niche-like microenvironment. This study carries significant implications for hPSC cultivation and encourages more in-depth studies towards the fundamentals of hPSC-CDM interactions. (C) 2017 Elsevier Ltd. All rights reserved.
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Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
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