Surfactant 4-nonylphenyl-polyethylene glycol stimulates reactive oxygen species generation and apoptosis in human neuroblastoma cells
- Authors
- Zerin, Tanana; Song, Hoyeon; Gil, Hyowook; Hong, Saeyong
- Issue Date
- 1-Mar-2017
- Publisher
- IOS Press
- Keywords
- Apoptosis Cytochrome c; Caspase-3/7; Caspase-9; Nonionic surfactant; Oxidative stress
- Citation
- Journal of Environmental Sciences, v.53, pp 262 - 268
- Pages
- 7
- Journal Title
- Journal of Environmental Sciences
- Volume
- 53
- Start Page
- 262
- End Page
- 268
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7700
- DOI
- 10.1016/j.jes.2016.04.011
- ISSN
- 1001-0742
1878-7320
- Abstract
- The formulation of most pesticides is proprietary and individual components are therefore not generally known. In a preliminary study, we identified six compounds that are often present in pesticides, of which 4-nonylphenyl-polyethylene glycol (NP-40) was found to be the most toxic. In this study, we investigated the toxicity of NP-40 and underlying mechanism in neuronal SK-N-SH cells. Exposure to NP 40 at concentrations higher than 60 mu mol/L for 24 hr decreased cell viability. The cytotoxicity of NP-40 was time- and concentration-dependent. Nuclear fragmentation and chromatin condensation were apparent starting at 50 mu mol/L NP-40, and increased at higher concentrations. The expression of apoptotic factors including p53 and B-cell lymphoma (Bcl)-2-associated X protein was upregulated, while that of the anti-apoptotic marker Bcl-2 was downregulated at 80 mu mol/L NP-40. Cytochrome c release was observed from 80 to 100 mu mol/L by confocal microscopy. Caspase-9 and -3/7 activities increased according to concentration, and fluorescence-activated cell sorting analysis showed that apoptosis was induced at 50 mu mol/L and was increased at 80 mu mol/L. Our findings indicate that NP-40 stimulates the mitochondrial-mediated apoptosis pathway and reactive oxygen species production in a concentration-dependent manner, and suggest that antioxidant administration may be an effective treatment for patients with acute NP-40 poisoning. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B. V.
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Collections - College of Medicine > Department of Internal Medicine > 1. Journal Articles
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