Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cellsopen access
- Authors
- Kim, Kyung-Hee; Yeo, Seung-Gu; Yoo, Byong Chul; Myung, Jae Kyung
- Issue Date
- 2-Feb-2017
- Publisher
- Public Library of Science
- Keywords
- calgranulin B; gastrointestinal cancer cells; proteins
- Citation
- PLoS ONE, v.12, no.2
- Journal Title
- PLoS ONE
- Volume
- 12
- Number
- 2
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7789
- DOI
- 10.1371/journal.pone.0171232
- ISSN
- 1932-6203
- Abstract
- Calgranulin B is known to be involved in tumor development, but the underlying molecular mechanism is not clear. To gain insight into possible roles of calgranulin B, we screened for calgranulin B-interacting molecules in the SNU-484 gastric cancer and the SNU-81 colon cancer cells. Calgranulin B-interacting partners were identified by yeast two-hybrid and functional information was obtained by computational analysis. Most of the calgranulin B interacting partners were involved in metabolic and cellular processes, and found to have molecular function of binding and catalytic activities. Interestingly, 46 molecules in the network of the calgranulin B-interacting proteins are known to be associated with cancer and FKBP2 was found to interact with calgranulin B in both SNU-484 and SNU-81 cells. Polyubiquitin-C encoded by UBC, which exhibited an interaction with calgranulin B, has been associated with various molecules of the extracellular space and plasma membrane identified in our screening, including Na-K-CI cotransporter 1 and dystonin in SNU-484 cells, and ATPase subunit beta-1 in SNU-81 cells. Our data provide novel insight into the roles of calgranulin B of gastrointestinal cancer cells, and offer new clues suggesting calgranulin B acts as an effector molecule through which the cell can communicate with the tumor microenvironment via polyubiquitin-C.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Radiation Oncology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/7789)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.