Augmenting invitro osteogenesis of a glycine-arginine-glycine-aspartic-conjugated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel composite and invivo bone biogenesis through stem cell delivery
- Authors
- Linh, Nguyen T. B.; Paul, Kallyanashis; Kim, Boram; Lee, Byong-Taek
- Issue Date
- Nov-2016
- Publisher
- SAGE Publications
- Keywords
- Glycine-arginine-glycine-aspartic; human adipose stem cell; osteogenesis; bone formation; biphasic calcium phosphate; oxidized alginate; gelatin
- Citation
- Journal of Biomaterials Applications, v.31, no.5, pp 661 - 673
- Pages
- 13
- Journal Title
- Journal of Biomaterials Applications
- Volume
- 31
- Number
- 5
- Start Page
- 661
- End Page
- 673
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8641
- DOI
- 10.1177/0885328216667633
- ISSN
- 0885-3282
1530-8022
- Abstract
- A functionally modified peptide-conjugated hydrogel system was fabricated with oxidized alginate/gelatin loaded with biphasic calcium phosphate to improve its biocompatibility and functionality. Sodium alginate was treated by controlled oxidation to transform the cis-diol group into an aldehyde group in a controlled manner, which was then conjugated to the amine terminus of glycine-arginine-glycine-aspartic. Oxidized alginate glycine-arginine-glycine-aspartic was then combined with gelatin-loaded biphasic calcium phosphate to form a hydrogel of composite oxidized alginate/gelatin/biphasic calcium phosphate that displayed enhanced human adipose stem cell adhesion, spreading and differentiation. H-1 nuclear magnetic resonance and electron spectroscopy for chemical analysis confirmed that the glycine-arginine-glycine-aspartic was successfully grafted to the oxidized alginate. Co-delivery of glycine-arginine-glycine-aspartic and human adipose stem cell in a hydrogel matrix was studied with the results indicating that hydrogel incorporated modified with glycine-arginine-glycine-aspartic and seeded with human adipose stem cell enhanced osteogenesis invitro and bone formation invivo.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Regenerative Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.