The protective role of phytochemicals on TiO2 nanoparticles-induced DNA damage in lymphocytes
- Authors
- Ryu, A-Reum; Bang, In-Chul; Lee, Seong-A; Lee, Mi-Young
- Issue Date
- Sep-2016
- Publisher
- Academy of Environmental Biology
- Keywords
- Comet assay; DNA damage; Lymphocyte; Phytochemical; TiO2-nanoparticles
- Citation
- Journal of Environmental Biology, v.37, no.5, pp 913 - 917
- Pages
- 5
- Journal Title
- Journal of Environmental Biology
- Volume
- 37
- Number
- 5
- Start Page
- 913
- End Page
- 917
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/8809
- ISSN
- 0254-8704
- Abstract
- The adverse health effect of nanoparticles is of concern for humans and environment. In the present study, TiO2-nanoparticles (TiO2-NPs)-induced oxidative DNA damage in lymphocytes was measured by comet assay. 80 mu g ml(-1) TiO2-NPs induced approximately 3-fold increase in DNA damage than in the PBS-control group as measured by olive tail moment. However, on treating vitamin C and N-acetylcysteine, DNA damage was effectively protected in a concentration dependent manner. Moreover, the protective effect of several phytochemicals including berberine, resveratrol, sulforaphane, and curcumin on DNA damage caused by TiO2-NPs was manifested. The increased olive tail moment induced by TiO2-NPs was effectively inhibited by treatment with these phytochemicals. Especially, olive tail moment of 5 mu g ml(-1) berberine-treated group was significantly reduced down to the level of control group, showing almost complete protection. Taken together, the protective effect of phytochemicals against DNA damage by TiO2-NPs may be applied for the development of antidote for TiO2 toxicity.
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Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
- College of Medical Sciences > Department of Occupational Therapy > 1. Journal Articles
- College of Natural Sciences > Department of Biology > 1. Journal Articles
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