Chemical-induced formation of BZ-junction with base extrusion
- Authors
- Subramani, VK[Subramani, Vinod Kumar]; Ravichandran, S[Ravichandran, Subramaniyam]; Bansal, V[Bansal, Varun]; Kim, KK[Kim, Kyeong Kyu]
- Issue Date
- 22-Jan-2019
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Z-DNA; BZ-Junction; Z-DNA junction; 2-Aminopurine; Base-extrusion; Z-inducer
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.508, no.4, pp.1215 - 1220
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 508
- Number
- 4
- Start Page
- 1215
- End Page
- 1220
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/11294
- DOI
- 10.1016/j.bbrc.2018.12.045
- ISSN
- 0006-291X
- Abstract
- The crystal structure of BZ junction reveals that left-handed Z-DNA stabilized by Z-DNA binding domain (Z alpha) is continuously stacked to right-handed B-DNA with AT bases' extrusion in the junction site. However, this structure might not fully represent the BZ-junction in solution due to the possibility of the junction formation either by crystal packing or Z alpha interaction. Therefore, we investigated BZ-junction in solution with chemical Z-DNA inducers using CD and 2-aminopurine base-extrusion assay. We confirmed the formation of Z-DNA and BZ-junction with base-extrusion by chemical Z-DNA inducers. However, neither typical Z-DNA nor base-extrusion could be detected with some inducers such as spermine, suggesting that the energy barrier for the formation of the BZ junction might vary depending on the Z-DNA induction conditions. (C) 2018 Elsevier Inc. All rights reserved.
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- Appears in
Collections - Medicine > Department of Medicine > 1. Journal Articles
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