RNA interference-mediated simultaneous silencing of four genes using cross-shaped RNA
- Authors
- Lee, TY[Lee, Tae Yeon]; Chang, CI[Chang, Chan Il]; Lee, D[Lee, Dooyoung]; Hong, SW[Hong, Sun Woo]; Shin, C[Shin, Chanseok]; Li, CJ[Li, Chiang J.]; Kim, S[Kim, Soyoun]; Haussecker, D[Haussecker, Dirk]; Lee, DK[Lee, Dong-ki]
- Issue Date
- Apr-2013
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- polyethyleneimine (PEI); quadruple interfering RNA
- Citation
- MOLECULES AND CELLS, v.35, no.4, pp.320 - 326
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MOLECULES AND CELLS
- Volume
- 35
- Number
- 4
- Start Page
- 320
- End Page
- 326
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/60981
- DOI
- 10.1007/s10059-013-2316-7
- ISSN
- 1016-8478
- Abstract
- The structural flexibility of RNA interference (RNAi)-triggering nucleic acids suggests that the design of unconventional RNAi trigger structures with novel features is possible. Here, we report a cross-shaped RNA duplex structure, termed quadruple interfering RNA (qiRNA), with multiple target gene silencing activity. qiRNA triggers the simultaneous down-regulation of four cellular target genes via an RNAi mechanism. In addition, qiRNA shows enhanced intracellular delivery and target gene silencing over conventional siRNA when complexed with jetPEI, a linear polyethyleneimine (PEI). We also show that the long antisense strand of qiRNA is incorporated intact into an RNA-induced silencing complex (RISC). This novel RNA scaffold further expands the repertoire of RNAi-triggering molecular structures and could be used in the development of therapeutics for various diseases including viral infections and cancer.
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- Appears in
Collections - Science > Department of Chemistry > 1. Journal Articles
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