N-Acetylcysteine and N-Nitroarginine Methyl Ester Attenuate Carboplatin-Induced Ototoxicity in Dissociated Spiral Ganglion Neuron Culturesopen access
- Authors
- Moon, IJ[Moon, Il Joon]; Kim, KR[Kim, Ki Ryung]; Chu, HS[Chu, Ho-Suk]; Kim, SH[Kim, Se Hyung]; Chung, WH[Chung, Won-Ho]; Cho, YS[Cho, Yang-Sun]; Hong, SH[Hong, Sung Hwa]
- Issue Date
- Mar-2011
- Citation
- CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.4, no.1, pp.11 - 17
- Indexed
- SCIE
SCOPUS
KCI
OTHER
- Journal Title
- CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY
- Volume
- 4
- Number
- 1
- Start Page
- 11
- End Page
- 17
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/70643
- DOI
- 10.3342/ceo.2011.4.1.11
- ISSN
- 1976-8710
- Abstract
- Objectives. Carboplatin, a platinum-containing anti-cancer drug used to treat a variety of cancers, induces ototoxicity. Since, reactive oxygen species (ROS) and nitric oxide (NO) seem to be responsible for this toxicity, the antioxidant, N-acetyl-L-cysteine (L-NAC), and NO synthetase inhibitor, N-nitro-L-arginine methyl ester (L-NAME) were predicted to have protective effects against carboplatin ototoxicity. The aim of this study was to test for the protective effects of L-NAC and L-NAME on cochlear hair cells and spiral ganglion neurons (SGNs). Methods. Cochlear organotypic cultures and dissociated spiral ganglion neuron cultures, from mice postnatal day 5 cultures were used in this study. The cultures were treated with carboplatin alone or in combination with L-NAC or L-NAME, and carboplatin-induced damage was monitored. Results. Treatment with carboplatin induced a significant loss of outer hair cells, while inner hair cells were preserved in the cochlear organotypic cultures. Addition of L-NAC or L-NAME reduced the amount of carboplatin-induced hair cell damage; the differences did not reach statistical significance. However, carboplatin significantly decreased the number of surviving SGNs in dissociated cultures. The toxic effects were significantly reduced by addition of L-NAC or L-NAME. In addition, carboplatin induced the loss of neurites from the SGN somata, and this was not blocked with L-NAC or L-NAME. Conclusion. The results of this study suggest that ROS and NO are involved in carboplatin-induced damage to hair cells and SGNs, and administration of L-NAC/L-NAME can be used to attenuate the toxicity.
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- Appears in
Collections - Medicine > Department of Medicine > 1. Journal Articles
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