Regulation of Blood Vessel versus Lymphatic Vessel Growth in the Cornea
- Authors
- Chung, ES[Chung, Eui-Sang]; Saban, DR[Saban, Daniel R.]; Chauhan, SK[Chauhan, Sunil K.]; Dana, R[Dana, Reza]
- Issue Date
- Apr-2009
- Publisher
- ASSOC RESEARCH VISION OPHTHALMOLOGY INC
- Citation
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v.50, no.4, pp.1613 - 1618
- Indexed
- SCIE
SCOPUS
- Journal Title
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
- Volume
- 50
- Number
- 4
- Start Page
- 1613
- End Page
- 1618
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/78179
- DOI
- 10.1167/iovs.08-2212
- ISSN
- 0146-0404
- Abstract
- PURPOSE. In the present study, the authors developed novel models to stimulate blood vessel formation (hemangiogenesis) versus lymphatic vessel formation (lymphangiogenesis) in the cornea. METHODS. Micropellets loaded with high-dose (80 ng) or low-dose (12.5 ng) basic fibroblast growth factor (bFGF) were placed in BALB/c corneas. Angiogenic responses were analyzed by immunohistochemistry to quantify blood neovessels (BVs) and lymphatic neovessels (LVs) to 3 weeks after implantation. Areas covered by BV and LV were calculated and expressed as a percentage of the total corneal area (percentage BV and percentage LV). Hemangiogenesis (HA) and lymphangiogenesis (LA) were also assessed after antibody blockade of VEGFR-2 or VEGFR-3 RESULTS. Although high-dose bFGF stimulation induced a more potent angiogenic response, the relative LV (RLV = percentage LV/percentage BV X 100) was nearly identical with high- and low-doses of bFGF. Delayed LA responses induced 3 weeks after implantation of high-dose bFGF resulted in a lymphatic vessel-dominant phenotype. Interestingly, the blockade of VEGFR-2 significantly suppressed BV and LV. However, the blockade of VEGFR-3 inhibited only LV (P = 0.0002) without concurrent inhibition of BV (P = 0.79), thereby resulting in a blood vessel-dominant phenotype CONCLUSIONS. An HA-dominant corneal phenotype can be obtained in BALB/c mice 2 weeks after implantation of an 80-ng bFGF micropellet with VEGFR-3 blockade. Alternatively, an LA-dominant corneal phenotype can be obtained 3 weeks after implantation of an 80-ng bFGF micropellet without supplementary modulating agents. These models will be useful in evaluating the differential contribution of BV and LV to a variety of corneal abnormalities, including transplant rejection, wound healing and microbial keratitis. (Invest Ophthalmol Vis Sci. 2009; 50: 1613-1618) DOI:10.1167/iovs.08-2212
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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