Topical application of galgeunhwanggeumhwangryeon-tang recovers skin-lipid barrier and ameliorates inflammation via filaggrin-thymic stromal lymphopoietin-interleukin 4 pathway
- Authors
- Ahn, S.-H.[Ahn, S.-H.]; Shin, S.[Shin, S.]; Do, Y.[Do, Y.]; Jo, Y.[Jo, Y.]; Ryu, D.[Ryu, D.]; Ha, K.-T.[Ha, K.-T.]; Kim, K.[Kim, K.]
- Issue Date
- Dec-2021
- Publisher
- MDPI
- Keywords
- Atopic dermatitis; Ceramide; Filaggrin; Galgeunhwanggeumhwangryeon-tang; IL-4
- Citation
- Medicina (Lithuania), v.57, no.12
- Indexed
- SCIE
SCOPUS
- Journal Title
- Medicina (Lithuania)
- Volume
- 57
- Number
- 12
- URI
- https://scholarworks.bwise.kr/skku/handle/2021.sw.skku/94611
- DOI
- 10.3390/medicina57121387
- ISSN
- 1010-660X
- Abstract
- Background and objectives: The purpose of this study was to confirm the effect of Galgeunhwanggeumhwangryeon-tang (GGRT) on the skin barrier integrity and inflammation in an atopic dermatitis-like animal model. Materials and Methods: The model was established using lipid barrier elimination (LBE) in BALB/c mice. Ceramide 3B, a control drug, and GGRT were applied to the skin of LBE mice. Gross observation and histological examination were combined with measurement of skin score, trans-epidermal water loss, and pH. The expression of filaggrin, kallikrein-related peptidase 7 (KLK7), protease-activated receptor-2 (PAR-2), thymic stromal lymphopoietin (TSLP), and interleukin 4 (IL-4) was examined. Results: The effect of GGRT on atopic dermatitis was estimated in silico using two individual gene sets of human atopic dermatitis. In animal experiments, GGRT treatment reduced atopic dermatitis-like symptoms, as confirmed via gross and histological observations, skin score, pH change, and trans-epidermal water loss. The expression level of filaggrin increased in the skin of GGRT-treated mice compared to that in the LBE group. The expression levels of KLK7, PAR2, TSLP, and IL-4 were decreased in GGRT-treated mice skin compared to those in LBE mice. Conclusions: We demonstrated that GGRT restored the skin barrier and reduced inflammatory reactions in a murine model of atopic dermatitis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Collections - Medicine > Department of Medicine > 1. Journal Articles
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