Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron
- Authors
- Cho, Jea-Won; Han, Byeong-Gu; Park, Sang Youn; Kim, Seung Jun; Kim, Myoung-Dong; Lee, Byung Il
- Issue Date
- Oct-2013
- Publisher
- WILEY-BLACKWELL
- Keywords
- Bacteroides thetaiotaomicron; BT0793; glucose isomerase; xylose isomerase
- Citation
- ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, v.69, pp.1127 - 1130
- Journal Title
- ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
- Volume
- 69
- Start Page
- 1127
- End Page
- 1130
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/11153
- DOI
- 10.1107/S1744309113023877
- ISSN
- 1744-3091
- Abstract
- Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10 angstrom resolution at 100 Kusing synchrotron X-rays. The crystal was found to belong to space group P1, with unit-cell parameters a = 96.3, b = 101.7, c = 108.3 angstrom, alpha = 82.8, beta = 68.2, gamma = 83.0 degrees. The asymmetric unit contained eight subunits of xylose isomerase with a crystal volume per protein weight (V-M) of 2.38 angstrom(3) Da(-1) and a solvent content of 48.3%.
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