Application of quantitative real-time PCR for quantification of Rhodococcus sp EH831 in a polyurethane biofilter
DC Field | Value | Language |
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dc.contributor.author | Lee, E. H. | - |
dc.contributor.author | Cho, K. S. | - |
dc.contributor.author | Ryu, H. W. | - |
dc.date.available | 2018-05-10T15:22:29Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2009-01 | - |
dc.identifier.issn | 0254-8704 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/15902 | - |
dc.description.abstract | Rhodococcus sp. EH831 is a microbial species that can degrade volatile organic compounds. We optimized a method for monitoring quantitative real-time PCR (qRT-PCR) of EH831 that was incorporated into a polyurethane (PU) biofilter When the genomic DNA of EH831 was directly extracted from a PU sample with immobilized EH831, the recovery efficiency was very low due to DNA absorption into the PU. DNA amplification during PCR was also inhibited by PU impurities. Therefore, a pre-treatment step was necessary. We successfully recovered cells from the PU by squeezing the matrix, adding sterilized water, and vortexing. The recovery efficiency ranged from 105 to 144%, and there was no statistically significant difference. We designed a novel TaqMan probe for EH831 and demonstrated its high specificity for EH831. The detection range for EH831 was 10(5)-10(11) CFU ml(-1). The method described in this study can be used to investigate the relationship between quantitative analysis of Rhodococcus sp. EH831 and PU biofilter performance. | - |
dc.publisher | TRIVENI ENTERPRISES | - |
dc.relation.isPartOf | JOURNAL OF ENVIRONMENTAL BIOLOGY | - |
dc.subject | TERT-BUTYL ETHER | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | DEGRADATION | - |
dc.subject | TOLUENE | - |
dc.subject | BIODEGRADATION | - |
dc.subject | XYLENE | - |
dc.subject | WATER | - |
dc.subject | SOIL | - |
dc.subject | BIOFILTRATION | - |
dc.subject | DIVERSITY | - |
dc.title | Application of quantitative real-time PCR for quantification of Rhodococcus sp EH831 in a polyurethane biofilter | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF ENVIRONMENTAL BIOLOGY, v.30, no.1, pp.155 - 159 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000262706900025 | - |
dc.identifier.scopusid | 2-s2.0-59349102118 | - |
dc.citation.endPage | 159 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 155 | - |
dc.citation.title | JOURNAL OF ENVIRONMENTAL BIOLOGY | - |
dc.citation.volume | 30 | - |
dc.contributor.affiliatedAuthor | Ryu, H. W. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Biofilter | - |
dc.subject.keywordAuthor | Rhodococcus sp. | - |
dc.subject.keywordAuthor | Polyurethane | - |
dc.subject.keywordAuthor | Quantitative real-time PCR | - |
dc.subject.keywordAuthor | Monitoring | - |
dc.subject.keywordPlus | TERT-BUTYL ETHER | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | TOLUENE | - |
dc.subject.keywordPlus | BIODEGRADATION | - |
dc.subject.keywordPlus | XYLENE | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | SOIL | - |
dc.subject.keywordPlus | BIOFILTRATION | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.description.journalRegisteredClass | scopus | - |
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