Application of quantitative real-time PCR for quantification of Rhodococcus sp EH831 in a polyurethane biofilter
- Authors
- Lee, E. H.; Cho, K. S.; Ryu, H. W.
- Issue Date
- Jan-2009
- Publisher
- TRIVENI ENTERPRISES
- Keywords
- Biofilter; Rhodococcus sp.; Polyurethane; Quantitative real-time PCR; Monitoring
- Citation
- JOURNAL OF ENVIRONMENTAL BIOLOGY, v.30, no.1, pp.155 - 159
- Journal Title
- JOURNAL OF ENVIRONMENTAL BIOLOGY
- Volume
- 30
- Number
- 1
- Start Page
- 155
- End Page
- 159
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/15902
- ISSN
- 0254-8704
- Abstract
- Rhodococcus sp. EH831 is a microbial species that can degrade volatile organic compounds. We optimized a method for monitoring quantitative real-time PCR (qRT-PCR) of EH831 that was incorporated into a polyurethane (PU) biofilter When the genomic DNA of EH831 was directly extracted from a PU sample with immobilized EH831, the recovery efficiency was very low due to DNA absorption into the PU. DNA amplification during PCR was also inhibited by PU impurities. Therefore, a pre-treatment step was necessary. We successfully recovered cells from the PU by squeezing the matrix, adding sterilized water, and vortexing. The recovery efficiency ranged from 105 to 144%, and there was no statistically significant difference. We designed a novel TaqMan probe for EH831 and demonstrated its high specificity for EH831. The detection range for EH831 was 10(5)-10(11) CFU ml(-1). The method described in this study can be used to investigate the relationship between quantitative analysis of Rhodococcus sp. EH831 and PU biofilter performance.
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Collections - College of Engineering > Department of Chemical Engineering > 1. Journal Articles
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