Blood-declustering excretable metal clusters assembled in DNA matrix
- Authors
- Lee, Jaiwoo; Le, Quoc-Viet; Ko, Seungbeom; Kang, Sungtaek; Macgregor, Robert B.; Shim, Gayong; Oh, Yu-Kyoung
- Issue Date
- Oct-2022
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Metal clusters; Blood-declustering; Excretable; Kidney distribution; Interparticle plasmon coupling effect
- Citation
- BIOMATERIALS, v.289
- Journal Title
- BIOMATERIALS
- Volume
- 289
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42958
- DOI
- 10.1016/j.biomaterials.2022.121754
- ISSN
- 0142-9612
- Abstract
- We report polymeric DNA-supported gold clusters that achieve interparticle plasmon-coupling, generate immunotherapeutic effects at the tumor tissue, but decluster in the bloodstream. As immunostimulating DNA, we used polyCpG DNA, which could act as a supporting matrix for metal clusters, enabling the clusters to decluster in the bloodstream. We constructed polyCpG-supported gold nanoclusters (AuPCN). For comparison with AuPCN, monomer CpG-bound gold nanoparticles (AuMC) were used. Unlike AuMC, AuPCN showed an inter-particle plasmon-coupling effect and a higher light-to heat conversion efficiency. In the serum, AuPCN declus-tered to subunits. The CT26 tumor rechallenge of mice pretreated with AuPCN(+NIR) was followed by 0% tumor recurrence and 100% survival for up to 80 days. Compared with other groups, AuPCN(+NIR)-treated mice revealed greater cytotoxic T cell-infiltration in distant tumors and higher memory T cells in the lymph nodes. Until 7 days post-dose, the urinary excretion of Au was observed in the AuPCN-treated group, but not in the Au nanoparticle-treated mice. Although we used gold clusters and concatemeric immunostimulatory CpG as com-ponents of AuPCN, the concept of declustering in the bloodstream can be applied to design other functional DNA scaffold-based metal clusters with reduced concerns for long-term retention in the body.
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