DNA-based artificial dendritic cells for in situ cytotoxic T cell stimulation and immunotherapyopen access
- Authors
- Quoc-Viet Le; Lee, Jaiwoo; Byun, Junho; Shim, Gayong; Oh, Yu-Kyoung
- Issue Date
- Sep-2022
- Publisher
- KEAI PUBLISHING LTD
- Keywords
- Artificial dendritic cell; DNA microflower; In situ T cell stimulation; Programmed T cell expansion; Immunotherapy
- Citation
- BIOACTIVE MATERIALS, v.15, pp.160 - 172
- Journal Title
- BIOACTIVE MATERIALS
- Volume
- 15
- Start Page
- 160
- End Page
- 172
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/42959
- DOI
- 10.1016/j.bioactmat.2021.12.001
- ISSN
- 2452-199X
- Abstract
- In immunotherapy, ex vivo stimulation of T cells requires significant resources and effort. Here, we report artificial dendritic cell-mimicking DNA microflowers (DM) for programming T cell stimulation in situ. To mimic dendritic cells, DNA-based artificial dendritic microflowers were constructed, surface-coated with polydopamine, and further modified with anti-CD3 and anti-CD28 antibodies to yield antibody-modified DM (DM-A). The porous structure of DM-A allowed entrapment of the T cell-stimulating cytokine, ineterleukin-2, yielding interleukin-2-loaded DM-A (DM-AI). For comparison, polystyrene microparticles coated with polydopamine and modified with anti-CD3 and anti-CD28 antibodies (PS-A) were used. Compared to PS-A, DM-AI showed significantly greater contact with T cell surfaces. DM-AI provided the highest ex vivo expansion of cytotoxic T cells. Local injection of DM-AI to tumor tissues induced the recruitment of T cells and expansion of cytotoxic T cells in tumor microenvironments. Unlike the other groups, model animals injected with DM-AI did not exhibit growth of primary tumors. Treatment of mice with DM-AI also protected against growth of a rechallenged distant tumor, and thus prevented tumor recurrence in this model. DM-AI has great potential for programmed stimulation of CD8(+) T cells. This concept could be broadly extended for the programming of specific T cell stimulation profiles.
- Files in This Item
-
Go to Link
- Appears in
Collections - College of Natural Sciences > School of Systems and Biomedical Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.