MicroRNA miR-263b-5p Regulates Developmental Growth and Cell Association by Suppressing Laminin A in Drosophilaopen access
- Authors
- Kim, Chae Jeong; Kim, Hyun Ho; Kim, Hee Kyung; Lee, Sojeong; Jang, Daegyu; Kim, Chanhyeok; Lim, Do-Hwan
- Issue Date
- Aug-2023
- Publisher
- MDPI
- Keywords
- Drosophila; miR-263b; Laminin A; extracellular matrix; basement membrane; tissue remodeling; fat body
- Citation
- BIOLOGY-BASEL, v.12, no.8
- Journal Title
- BIOLOGY-BASEL
- Volume
- 12
- Number
- 8
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/44276
- DOI
- 10.3390/biology12081096
- ISSN
- 2079-7737
- Abstract
- Basement membranes (BMs) play important roles under various physiological conditions in animals, including ecdysozoans. During development, BMs undergo alterations through diverse intrinsic and extrinsic regulatory mechanisms; however, the full complement of pathways controlling these changes remain unclear. Here, we found that fat body-overexpression of Drosophila miR-263b, which is highly expressed during the larval-to-pupal transition, resulted in a decrease in the overall size of the larval fat body, and ultimately, in a severe growth defect accompanied by a reduction in cell proliferation and cell size. Interestingly, we further observed that a large proportion of the larval fat body cells were prematurely disassociated from each other. Moreover, we present evidence that miR-263b-5p suppresses the main component of BMs, Laminin A (LanA). Through experiments using RNA interference (RNAi) of LanA, we found that its depletion phenocopied the effects in miR-263b-overexpressing flies. Overall, our findings suggest a potential role for miR-263b in developmental growth and cell association by suppressing LanA expression in the Drosophila fat body.
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