Ecdysone-induced microRNA miR-276a-3p controls developmental growth by targeting the insulin-like receptor in Drosophila
- Authors
- Lee, Sojeong; Kim, Nayeon; Jang, Daegyu; Kim, Hee Kyung; Kim, Jongin; Jeon, Ji Won; Lim, Do-Hwan
- Issue Date
- Dec-2023
- Publisher
- WILEY
- Keywords
- Drosophila; ecdysone; insulin-like receptor; miR-276a-3p
- Citation
- INSECT MOLECULAR BIOLOGY, v.32, no.6, pp.703 - 715
- Journal Title
- INSECT MOLECULAR BIOLOGY
- Volume
- 32
- Number
- 6
- Start Page
- 703
- End Page
- 715
- URI
- https://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/44637
- DOI
- 10.1111/imb.12872
- ISSN
- 0962-1075
- Abstract
- Animal growth is controlled by a variety of external and internal factors during development. The steroid hormone ecdysone plays a critical role in insect development by regulating the expression of various genes. In this study, we found that fat body-specific expression of miR-276a, an ecdysone-responsive microRNA (miRNA), led to a decrease in the total mass of the larval fat body, resulting in significant growth reduction in Drosophila. Changes in miR-276a expression also affected the proliferation of Drosophila S2 cells. Furthermore, we found that the insulin-like receptor (InR) is a biologically relevant target gene regulated by miR-276a-3p. In addition, we found that miR-276a-3p is upregulated by the canonical ecdysone signalling pathway involving the ecdysone receptor and broad complex. A reduction in cell proliferation caused by ecdysone was compromised by blocking miR-276a-3p activity. Thus, our results suggest that miR-276a-3p is involved in ecdysone-mediated growth reduction by controlling InR expression in the insulin signalling pathway.
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