Development of highly sensitive plasmonic biosensors encoded with gold nanoparticles on M13 bacteriophage networks
- Authors
- Moon, Joung-Il; Choi, Eun Jung; Joung, Younju; Oh, Jin-Woo; Joo, Sang-Woo; Choo, Jaebum
- Issue Date
- Feb-2024
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- Surface-enhanced Raman scattering; M13 bacteriophage; Network assembly; SERS substrate; Nanogap
- Citation
- SENSORS AND ACTUATORS B-CHEMICAL, v.400
- Journal Title
- SENSORS AND ACTUATORS B-CHEMICAL
- Volume
- 400
- URI
- https://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/49001
- DOI
- 10.1016/j.snb.2023.134916
- ISSN
- 0925-4005
0925-4005
- Abstract
- In this study, a novel nanoplasmonic substrate is developed for biomedical applications. In nanoplasmonic biosensors, a substrate that can induce strong hot spots by forming uniform nanogaps smaller than 10 nm is required to implement a device capable of detecting biomarkers at low concentrations. Herein, gold nanoparticles-encoded M13 bacteriophage (AuNPs@M13) substrate was developed by immobilizing 60 nm AuNPs onto the surface of an M13 bacteriophage scaffold with a network assembly structure. The plasmonic characteristics of the proposed AuNPs-M13 were evaluated by assessing the nanogap distribution, electromagnetic enhancement effects, and reproducibility of the fabricated substrate. Additionally, an immunoassay for severe acute respiratory syndrome coronavirus 2 was performed to confirm the clinical applicability of the proposed AuNPs@M13 substrate. When the AuNPs@M13 was used as a nanoplasmonic substrate, it was possible to achieve better sensitivity (limit of detection [LoD] = 1.21 PFU/mL) than that with the commercially available lateral flow assay strip (LoD = 300-500 PFU/mL) or enzyme-linked immunoassay (LoD = 48 PFU/mL). The proposed AuNPs@M13 in this study is expected to be used as an innovative biomedical device for the highsensitivity detection of various biomarkers.
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