Monitoring disease-implicated hydrogen sulfide in blood using a facile deproteinization-based electrochemiluminescence chemosensor system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bak, Yecheol | - |
dc.contributor.author | Anjong, Tikum Florence | - |
dc.contributor.author | Park, Jihwan | - |
dc.contributor.author | Jeon, Sang Hyeon | - |
dc.contributor.author | Kim, Jihwan | - |
dc.contributor.author | Lee, Kangwon | - |
dc.contributor.author | Shin, Iksoo | - |
dc.contributor.author | Kim, Sehoon | - |
dc.date.accessioned | 2024-02-22T05:30:18Z | - |
dc.date.available | 2024-02-22T05:30:18Z | - |
dc.date.issued | 2023-11 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/49195 | - |
dc.description.abstract | Hydrogen sulfide (H2S), an antioxidant and cell signaling molecule, plays a critical role in redox reactions in the biological system, and its imbalances in blood levels have been implicated in various diseases. Here, we demonstrate a novel chemosensor-based "turn-on" electrochemiluminescence (ECL) system for the selective and sensitive detection of H2S in a type 1 diabetes rat model. Our luminogenic chemosensor (Probe) is a redox-stable cyclometalating iridium (III) complex with emission-quenching 2,4-dinitrophenyl ether (DNP) that shows a significant enhancement in emission intensity upon cleavage of DNP by selective reaction towards H2S. To achieve robust and reliable ECL emission of Probe for biological analysis, we developed a facile deproteinization protocol based on organic solvent-induced protein precipitation/syringe filtration that eliminates uncontrolled protein adsorption on the electrode surface from protein-rich biological samples. Our deproteinization-assisted ECL chemosensor system showed effectiveness in detecting disease-associated H2S levels in biological media with a linear response in the range of 0-250 mu M (R-2: 0.992). It enables reliable in vitro monitoring of H2S level changes in blood samples of a type 1 diabetes rat model (P = 0.0296). This strategy to integrate chemosensor-based ECL and facile deproteinization protocol provides a sensitive and rapid disease-monitoring platform for detecting small-molecule biomarkers. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | Monitoring disease-implicated hydrogen sulfide in blood using a facile deproteinization-based electrochemiluminescence chemosensor system | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.snb.2023.134364 | - |
dc.identifier.bibliographicCitation | SENSORS AND ACTUATORS B-CHEMICAL, v.394 | - |
dc.identifier.wosid | 001144437900001 | - |
dc.identifier.scopusid | 2-s2.0-85166193436 | - |
dc.citation.title | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.volume | 394 | - |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S0925400523010791 | - |
dc.publisher.location | 스위스 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordAuthor | Electrochmiluminescence | - |
dc.subject.keywordAuthor | Chemosensor | - |
dc.subject.keywordAuthor | Hydrogen sulfide | - |
dc.subject.keywordAuthor | Small molecule biomarker | - |
dc.subject.keywordAuthor | Type 1 diabetes | - |
dc.subject.keywordPlus | FLUORESCENT-PROBE | - |
dc.subject.keywordPlus | QUANTITATIVE DETECTION | - |
dc.subject.keywordPlus | PROTEIN ADSORPTION | - |
dc.subject.keywordPlus | SIGNALING MOLECULE | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | QUANTUM DOTS | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | GASOTRANSMITTER | - |
dc.subject.keywordPlus | STREPTOZOTOCIN | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Soongsil University Library 369 Sangdo-Ro, Dongjak-Gu, Seoul, Korea (06978)02-820-0733
COPYRIGHT ⓒ SOONGSIL UNIVERSITY, ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.