Monitoring disease-implicated hydrogen sulfide in blood using a facile deproteinization-based electrochemiluminescence chemosensor systemopen access
- Authors
- Bak, Yecheol; Anjong, Tikum Florence; Park, Jihwan; Jeon, Sang Hyeon; Kim, Jihwan; Lee, Kangwon; Shin, Iksoo; Kim, Sehoon
- Issue Date
- Nov-2023
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- Electrochmiluminescence; Chemosensor; Hydrogen sulfide; Small molecule biomarker; Type 1 diabetes
- Citation
- SENSORS AND ACTUATORS B-CHEMICAL, v.394
- Journal Title
- SENSORS AND ACTUATORS B-CHEMICAL
- Volume
- 394
- URI
- https://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/49195
- DOI
- 10.1016/j.snb.2023.134364
- ISSN
- 0925-4005
0925-4005
- Abstract
- Hydrogen sulfide (H2S), an antioxidant and cell signaling molecule, plays a critical role in redox reactions in the biological system, and its imbalances in blood levels have been implicated in various diseases. Here, we demonstrate a novel chemosensor-based "turn-on" electrochemiluminescence (ECL) system for the selective and sensitive detection of H2S in a type 1 diabetes rat model. Our luminogenic chemosensor (Probe) is a redox-stable cyclometalating iridium (III) complex with emission-quenching 2,4-dinitrophenyl ether (DNP) that shows a significant enhancement in emission intensity upon cleavage of DNP by selective reaction towards H2S. To achieve robust and reliable ECL emission of Probe for biological analysis, we developed a facile deproteinization protocol based on organic solvent-induced protein precipitation/syringe filtration that eliminates uncontrolled protein adsorption on the electrode surface from protein-rich biological samples. Our deproteinization-assisted ECL chemosensor system showed effectiveness in detecting disease-associated H2S levels in biological media with a linear response in the range of 0-250 mu M (R-2: 0.992). It enables reliable in vitro monitoring of H2S level changes in blood samples of a type 1 diabetes rat model (P = 0.0296). This strategy to integrate chemosensor-based ECL and facile deproteinization protocol provides a sensitive and rapid disease-monitoring platform for detecting small-molecule biomarkers.
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