Transcriptional Downregulation by Nucleotide Substitution with the Minor Allele of rs3760776 Located in the Promoter of FUT6 Gene
DC Field | Value | Language |
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dc.contributor.author | Ryoo, Hyunju | - |
dc.contributor.author | Ryu, Jihye | - |
dc.contributor.author | Lee, Chaeyoung | - |
dc.date.available | 2018-05-09T07:34:53Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2015-06 | - |
dc.identifier.issn | 0006-2928 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8715 | - |
dc.description.abstract | We examined the promoter activity of an association signal in an upstream region of the gene encoding fucosyltransferae 6 (FUT6) identified from a recent genomewide association study for the N-glycan level. The luciferase assay using reporter constructs with T and C alleles at rs3760776 revealed differential promoter activity. The amount of luciferin expressed with the C allele corresponded to that without the reporter construct (P > 0.05). On the other hand, the expression was dramatically reduced with the T allele (P < 0.05). The difference in transcriptional activity between the two alleles was confirmed by an electrophoretic mobility shift assay. It demonstrated that the promoter with a T allele had a stronger binding affinity to nuclear factors than that with the C allele. We concluded that the T allele of rs3760776 might repress the transcription of the FUT6 gene. Further studies are warranted to understand its underlying mechanism and its influence on susceptibility to potential diseases. | - |
dc.publisher | SPRINGER/PLENUM PUBLISHERS | - |
dc.relation.isPartOf | BIOCHEMICAL GENETICS | - |
dc.subject | HEPATOCELLULAR-CARCINOMA CELLS | - |
dc.subject | FUCOSYL-TRANSFERASE | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | EXPRESSION | - |
dc.subject | VI | - |
dc.title | Transcriptional Downregulation by Nucleotide Substitution with the Minor Allele of rs3760776 Located in the Promoter of FUT6 Gene | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s10528-015-9673-1 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL GENETICS, v.53, no.4-6, pp.72 - 78 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000355316400002 | - |
dc.identifier.scopusid | 2-s2.0-84929958159 | - |
dc.citation.endPage | 78 | - |
dc.citation.number | 4-6 | - |
dc.citation.startPage | 72 | - |
dc.citation.title | BIOCHEMICAL GENETICS | - |
dc.citation.volume | 53 | - |
dc.contributor.affiliatedAuthor | Ryu, Jihye | - |
dc.contributor.affiliatedAuthor | Lee, Chaeyoung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Electrophoretic mobility shift assay | - |
dc.subject.keywordAuthor | FUT6 | - |
dc.subject.keywordAuthor | N-glycan | - |
dc.subject.keywordAuthor | Promoter activity | - |
dc.subject.keywordAuthor | Single nucleotide variant | - |
dc.subject.keywordPlus | HEPATOCELLULAR-CARCINOMA CELLS | - |
dc.subject.keywordPlus | FUCOSYL-TRANSFERASE | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | VI | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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