Transcriptional Downregulation by Nucleotide Substitution with the Minor Allele of rs3760776 Located in the Promoter of FUT6 Gene
- Authors
- Ryoo, Hyunju; Ryu, Jihye; Lee, Chaeyoung
- Issue Date
- Jun-2015
- Publisher
- SPRINGER/PLENUM PUBLISHERS
- Keywords
- Electrophoretic mobility shift assay; FUT6; N-glycan; Promoter activity; Single nucleotide variant
- Citation
- BIOCHEMICAL GENETICS, v.53, no.4-6, pp.72 - 78
- Journal Title
- BIOCHEMICAL GENETICS
- Volume
- 53
- Number
- 4-6
- Start Page
- 72
- End Page
- 78
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8715
- DOI
- 10.1007/s10528-015-9673-1
- ISSN
- 0006-2928
- Abstract
- We examined the promoter activity of an association signal in an upstream region of the gene encoding fucosyltransferae 6 (FUT6) identified from a recent genomewide association study for the N-glycan level. The luciferase assay using reporter constructs with T and C alleles at rs3760776 revealed differential promoter activity. The amount of luciferin expressed with the C allele corresponded to that without the reporter construct (P > 0.05). On the other hand, the expression was dramatically reduced with the T allele (P < 0.05). The difference in transcriptional activity between the two alleles was confirmed by an electrophoretic mobility shift assay. It demonstrated that the promoter with a T allele had a stronger binding affinity to nuclear factors than that with the C allele. We concluded that the T allele of rs3760776 might repress the transcription of the FUT6 gene. Further studies are warranted to understand its underlying mechanism and its influence on susceptibility to potential diseases.
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Collections - College of Natural Sciences > School of Systems and Biomedical Science > 1. Journal Articles
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