Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats
- Authors
- Joo, Minjae; Kim, Han Sang; Kwon, Tae Loon; Palikhe, Alisha; Zaw, Tin Sandar; Jeong, Ji Hoon; Sohn, Uy Dong
- Issue Date
- Jan-2015
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Colitis; Flavonoids; Inflammation; Quercetin; Reactive oxygen species
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.19, no.1, pp 43 - 50
- Pages
- 8
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 19
- Number
- 1
- Start Page
- 43
- End Page
- 50
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/10023
- DOI
- 10.4196/kjpp.2015.19.1.43
- ISSN
- 1226-4512
2093-3827
- Abstract
- It has been shown that the extracts including eupatilin and quercetin-3- beta -D-glucuronopyranoside had mucoprotective effects on the esophagus and stomach through their antioxidant activities. This study was designed to investigate the anti-inflammatory effect of these flavonoid compounds in an animal model of inflammatory bowel disease induced by 2,4,6-trinitrobenzene sulfonic acid. Experimental colitis was induced by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid. Extracts including eupatilin or quercetin-3- beta -D-glucuronopyranoside were orally administered to animals 48, 24, and 1 h prior to the induction of colitis and then again 24 h later. The animals were sacrificed 48 h after by 2,4,6-trinitrobenzene sulfonic acid treatment and the macroscopic appearance of the colonic lesions was scored in a blinded manner on a scale of 1 to 10. The inflammatory response to colitis induction was assessed by measuring myeloperoxidase activity, nitric oxide production, tumor necrosis factor- alpha expression, total glutathione levels, and malondialdehyde concentrations in the colon. The results indicated that extracts including eupatilin and extracts including quercetin-3- beta -D-glucuronopyranoside dose-dependently improved the morphology of the lesions induced by 2,4,6-trinitrobenzene sulfonic acid and reduced the ulcer index accordingly. In addition, rats receiving extracts including eupatilin and extracts including quercetin-3-,beta -D-glucuronopyranoside showed significantly decreased levels of mucosal myeloperoxidase activity, nitric oxide production, tumor necrosis factor- alpha expression, and malondialdehyde levels, and increased total glutathione levels. Extracts including eupatilin and extracts including quercetin-3- beta -D-glucuronopyranoside ameliorated the inflammatory response and colonic injury in acute colitis by decreasing oxidative stress and neutrophil activation. Extracts including eupatilin and extracts including quercetin-3- beta -D-glucuronopyranoside may inhibit acute colitis.
- Files in This Item
-
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
- College of Medicine > College of Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.