Gold nanoparticle-DNA aptamer composites as a universal carrier for in vivo delivery of biologically functional proteins
- Authors
- Ryou, Sang-Mi; Yeom, Ji-Hyun; Kang, Hyo Jung; Won, Miae; Kim, Jin-Sik; Lee, Boeun; Seong, Maeng-Je; Ha, Nam-Chul; Bae, Jeehyeon; Lee, Kangseok
- Issue Date
- Dec-2014
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Aptamer; Gold nanoparticle; Protein delivery; Targeted delivery
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.196, pp 287 - 294
- Pages
- 8
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 196
- Start Page
- 287
- End Page
- 294
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11476
- DOI
- 10.1016/j.jconrel.2014.10.021
- ISSN
- 0168-3659
1873-4995
- Abstract
- Although the delivery of biologically functional protein(s) into mammalian cells could be of tremendous value to biomedical research, the development of such technology has been hindered by the lack of a safe and effective delivery method. Here, we present a simple, efficient, and versatile gold nanoparticle-DNA aptamer conjugate (AuNP-Apt)-based system, with nanoblock-like properties, that allows any recombinant protein to be loaded without additional modifications and delivered into mammalian living systems. AuNP-Apt-based protein delivery system was able to deliver various proteins into variety of cell types in vitro without showing cytotoxicity. This AuNP-Apt system was also effective for the local and systemic targeted delivery of proteins in vivo. A local injection of the AuNP-Apt loaded with the apoptosis-inducing BIM protein efficiently inhibited the growth of xenograft tumors in mice. Furthermore, an intravenous injection of AuNP-Apt loaded with both epidermal growth factor (EGF) and BIM resulted in the targeted delivery of BIM into a xenograft tumor derived from EGF receptor-overexpressing cancer cells with no detectable systemic toxicity. Our findings show that this system can serve as an innovative platform for the development of protein-based biomedical applications. (C) 2014 Elsevier B.V. All rights reserved.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
- College of Natural Sciences > Department of Physics > 1. Journal Articles
- College of Natural Sciences > Department of Life Science > 1. Journal Articles
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