Signal Enhancement for Ultrasensitive Detection of Versatile Biomaterials Using Specific Aptamer Modified Au Nano-Complex
- Authors
- Choi, Jin-Ha; Jang, Hye-Mi; Mo, Kyosung; Um, Soong Ho; Min, Junhong; Oh, Byung-Keun
- Issue Date
- Nov-2014
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Aptamer; Immuno-PCR; Biosensor; Au Nanoparticle
- Citation
- SCIENCE OF ADVANCED MATERIALS, v.6, no.11, pp 2547 - 2553
- Pages
- 7
- Journal Title
- SCIENCE OF ADVANCED MATERIALS
- Volume
- 6
- Number
- 11
- Start Page
- 2547
- End Page
- 2553
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11695
- DOI
- 10.1166/sam.2014.2239
- ISSN
- 1947-2935
1947-2943
- Abstract
- For early diagnosis of several diseases, highly sensitive detection technology is necessary to analyze biomarkers quantitatively at low concentrations. To develop a sensitive detection system, a well-designed probe is very critical, and it mainly consisted of several bio-and nanomaterials, such as gold (Au) and silica nanoparticles. In this study, an ultrasensitive nanoprobe for the detection of versatile biomolecules was developed by the sandwich immuno-reaction technique. The developed nanoprobe was composed of aptamer-functionalized Au nanoparticles and magnetic microparticles (MMPs). In order to enhance the detection ability of the Au probe, the orientation of antibodies was defined using aptamer-induced specific binding to the F-c region of the antibody. And target antigens could be detected by the real-time polymerase chain reaction (RT-PCR) method, which was able to measure the concentration of aptamH7 (E. coli O157:H7). This system can be applied to the diagnosis of several diseases and environmental toxicity testing, especially for the detection of very low concentrations.
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