Specific rare cell capture using micro-patterned silicon nanowire platform
- Authors
- Lee, Sang-Kwon; Kim, Dong-Joo; Lee, GeeHee; Kim, Gil-Sung; Kwak, Minsuk; Fan, Rong
- Issue Date
- Apr-2014
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Cell capture; Circulating tumor cells (CTCs); Streptavidin functionalization; Silicon nanowires; Biocompatibility; Filopodia
- Citation
- BIOSENSORS & BIOELECTRONICS, v.54, pp 181 - 188
- Pages
- 8
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 54
- Start Page
- 181
- End Page
- 188
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12319
- DOI
- 10.1016/j.bios.2013.10.048
- ISSN
- 0956-5663
1873-4235
- Abstract
- We report on the rapid and direct quantification of specific cell captures using a micro-patterned streptavidin (STR)-functionalized silicon nanowire (SiNW) platform, which was prepared by Ag-assisted wet chemical etching and a photo-lithography process. This platform operates by high-affinity cell capture rendered by the combination of antibody-epithelial cell surface-binding, biotin-streptavidin binding, and the topologically enhanced cell-substrate interaction on a 3-dimensional SiNWs array. In this work, we developed a micro-patterned nanowire platform, with which we were able to directly evaluate the performance enhancement due to nanotopography. An excellent capture efficiency of similar to 96.6 +/- 6.7%, which is the highest value achieved thus far for the targeting specific A549 cells on a selective area of patterned SiNWs, is demonstrated. Direct comparison between the nanowire region and the planar region on the same substrate indicates dramatically elevated cell-capture efficiency on nanotopological surface identical surface chemistry (< 2% cell-capture efficiency). An excellent linear response was seen for quantifying captured A549 cells with respect to loaded cells. This study suggests that the micro-patterned STR-functionalized SiNWs platform provides additional advantage for detecting rare cells populations in a more quantitative and specific manner. (C) 2013 Elsevier B.V. All rights reserved.
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