Comparative Analysis of Flavonoids and Polar Metabolite Profiling of Tanno-Original and Tanno-High Rutin Buckwheat
- Authors
- Li, Xiaohua; Kim, Jae Kwang; Park, Soo-Yun; Zhao, Shicheng; Kim, Yeon Bok; Lee, Sanghyun; Park, Sang Un
- Issue Date
- Mar-2014
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Fagopyrum esculentum; flavonoid biosynthetic pathway; gene expression; rutin biosynthesis
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.62, no.12, pp 2701 - 2708
- Pages
- 8
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 62
- Number
- 12
- Start Page
- 2701
- End Page
- 2708
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12389
- DOI
- 10.1021/jf4049534
- ISSN
- 0021-8561
1520-5118
- Abstract
- Rutin is an important indicator for evaluating the quality of buckwheat. In this study, flavonoid biosynthesis was compared between two common cultivars (an original and a high-rutin line) of buckwheat, Fagopyrum esculentum Moench. Transcriptional levels of the main flavonoid biosynthetic genes were analyzed by real-time PCR, and main flavonoid metabolites were detected by high-performance liquid chromatography (HPLC); levels of gene expression varied among organs of the two cultivars. Significantly higher transcription levels of most flavonoid biosynthetic genes, except FeFLS1, were detected in stems of the high-rutin line than in stems of the original line. FeCH1 and FeFLS2 genes also showed higher expression levels in seeds of the high-rutin cultivar. In contrast, FePAL, FeC4H, Fe4CL1, FeCHS, FeF3H, FeF3'H, FeFLS2, and FeDFR were highly detected in the roots of the original line. The HPLC results indicated 1.73-, 1.62-, and 1.77-fold higher accumulation of rutin (the primary flavonoid compound) in leaves, stems, and mature seeds of the high-rutin cultivar (24.86, 1.46, and 1.36 mu g/mg, respectively) compared with the original cultivar (14.40, 0.90, and 0.77 mu g/mg, respectively). A total of 46 metabolites were identified from seeds by gas chromatography-time-of-flight mass spectrometry. The metabolite profiles were subjected to principal component analysis (PCA). PCA could clearly differentiate the original and high-rutin cultivars. Our results indicate that the high-rutin cultivar could be an excellent alternative for buckwheat culture, and we provide useful information for obtaining this cultivar.
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