Hizikia fusiformis fractions successfully improve atopic dermatitis indices in anti-CD3-stimulated splenocytes and 2,4-dinitrochlorobenzene-treated BALB/c mice
- Authors
- Lee, Kyu Ho; Kim, Hee Jung; Kim, Hae Bok; Kim, Seung Tae; Choi, Young Ri; Seo, Da Woom; Yu, Jung Min; Jang, Su Kil; Kim, Sang Moo; Lee, Do-Ik; Joo, Seong Soo
- Issue Date
- Mar-2014
- Publisher
- WILEY
- Keywords
- 2,4-dinitrochlorobenzene; atopic dermatitis; butanoic acid; helper T cell; Hizikia fusiformis
- Citation
- JOURNAL OF PHARMACY AND PHARMACOLOGY, v.66, no.3, pp 466 - 476
- Pages
- 11
- Journal Title
- JOURNAL OF PHARMACY AND PHARMACOLOGY
- Volume
- 66
- Number
- 3
- Start Page
- 466
- End Page
- 476
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12433
- DOI
- 10.1111/jphp.12179
- ISSN
- 0022-3573
2042-7158
- Abstract
- ObjectivesIn the present study, we aimed to examine whether fractions from an edible sea weed, Hizikia fusiformis, had immunomodulatory effects, particularly an anti-atopic effect, by attenuating the expression of T cell-dependent cytokines using in-vitro and in-vivo animal atopic dermatitis-like models. MethodsThe anti-atopic activities were examined in in vitro, and a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like mouse model using quantitative real-time polymerase chain reaction, electrophoretic-mobility shift and histophathological analysis. Key findingsOur results showed that the final fraction (F2) of H. fusiformis contained a higher amount of butanoic acid which was not found in the other fractions, and effectively inhibited T cell activation by inhibiting dephosphorylation of nuclear factor of activated T cells in electrophoretic-mobility shift assay. As a consequence, helper T cell-dependent cytokines, such as interleukin-2, -4 and interferon-, were significantly inhibited while activated with an anti-CD3 antibody. We also showed that skin challenged with DNCB successfully recovered when treated with 2.5 mg/kg, comparable to that by 0.25% prednicarbate. These results indicate that F2 may contribute to inhibit T cell activation by eliminating Th cell-dependent cytokines. ConclusionsTaken together, we concluded that F2 containing butanoic acid may be a new functional anti-atopic candidate, which probably acts through nuclear factor of activated T cell inactivation mechanisms.
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