Effect of Rice Cell-Derived Human Granulocyte-Macrophage Colony-Stimulating Factor on 5-Fluorouracil-Induced Mucositis in Hamsters
- Authors
- Won, Jong Hoon; Ji, Jung Eun; Ahn, Kyong Hoon; Kim, Seok Kyun; Choi, Jong Min; Ha, Hae Chan; Kim, Hye Mi; Yun, Chang Koo; Han, Kyuboem; Kim, Dae Kyong
- Issue Date
- Mar-2013
- Publisher
- PHARMACEUTICAL SOC JAPAN
- Keywords
- granulocyte-macrophage colony-stimulating factor; mucositis; wound healing; hamster buccal pouch; plant-based expression system
- Citation
- BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.36, no.3, pp 425 - 431
- Pages
- 7
- Journal Title
- BIOLOGICAL & PHARMACEUTICAL BULLETIN
- Volume
- 36
- Number
- 3
- Start Page
- 425
- End Page
- 431
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14800
- DOI
- 10.1248/bpb.b12-00869
- ISSN
- 0918-6158
1347-5215
- Abstract
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an important regulator of the maturation and function of cells in the granulocyte and macrophage lineages, and also plays a significant role in wound healing. In a previous study, we expressed human GM-CSF in rice cells (rice cell-derived human GM-CSF; rhGM-CSF). The purpose of the present study was to evaluate its effect on wound healing in oral mucositis. Oral mucositis was induced in Syrian hamster cheek pouches by 5-fluorouracil treatment and mechanical scratching. Ulcerated areas were treated from days 3 to 14 with an application of 200 mu L saline, or of the same volume of a solution containing 0.04, 0.2, or 1 mu g/mL rhGM-CSF. Treatment of hamsters with rhGM-CSF reduced the ulcerated areas of the oral mucosa, compared with the control. Early in the healing process, the mucositis tissue layer of the rhGM-CSF-treated group showed significantly decreased myeloper-oxidase activity and increased numbers of proliferating cell nuclear antigen (PCNA)-positive cells. Treatment with rhGM-CSF also affected expression of inflammatory cytokines in the ulcerative mucosal tissue. These results demonstrate the efficacy of plant-produced rhGM-CSF in wound healing and have significant implications for the development of rhGM-CSF as a therapeutic agent for ulcerative oral mucositis.
- Files in This Item
-
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.