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Preparation of photoreactive azidophenyl hyaluronic acid derivative: Protein immobilization for medical applications
- Authors
- Lee, Hyung-Jae; Park, Shin-Hye; Seo, Si-Yoong; Cho, Young-Min; Woo, Hee Dong; Ito, Yoshihiro; Son, Tae-Il
- Issue Date
- Feb-2013
- Publisher
- POLYMER SOC KOREA
- Keywords
- hyaluronic acid; 4-azidoaniline; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; photoreactive; protein immobilization
- Citation
- MACROMOLECULAR RESEARCH, v.21, no.2, pp 216 - 220
- Pages
- 5
- Journal Title
- MACROMOLECULAR RESEARCH
- Volume
- 21
- Number
- 2
- Start Page
- 216
- End Page
- 220
- ISSN
- 1598-5032
2092-7673
- Abstract
- Photoreactive azidophenyl hyaluronic acid (Az-HA) derivative was synthesized to immobilize proteins such as epidermal growth factor (EGF) and bone morphogenetic protein (BMP). The photosensitizing group, 4-azidoaniline, was introduced to the carboxyl group of hyaluronic acid by a water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). To characterize the synthesized Az-HA, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (H-1 NMR) and ultraviolet/visible (UV/vis) absorbance spectroscopy were performed. We demonstrated protein immobilization by using fluorescein isothiocyanate conjugated albumin (FITCBSA) on Az-HA derivative surfaces that were patterned with micro scale. Cytotoxicity and relative cell proliferation tests of the Az-HA derivative were performed on an MG-63 human osteoblast cell line for biocompatibility.
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