Sperm Penetration Assay as an Indicator of Bull Fertility
- Authors
- Park, Yoo-Jin; Mohamed, El-Sayed A.; Oh, Shin-Ae; Yoon, Sung-Jae; Kwon, Woo-Sung; Kim, Heung-Ruil; Lee, Myeung-Sik; Lee, Kichoon; Pang, Myung-Geol
- Issue Date
- Aug-2012
- Publisher
- SOCIETY REPRODUCTION & DEVELOPMENT-SRD
- Keywords
- Bull; Male fertility; Sperm; Sperm penetration assay; Prediction
- Citation
- JOURNAL OF REPRODUCTION AND DEVELOPMENT, v.58, no.4, pp 461 - 466
- Pages
- 6
- Journal Title
- JOURNAL OF REPRODUCTION AND DEVELOPMENT
- Volume
- 58
- Number
- 4
- Start Page
- 461
- End Page
- 466
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/15218
- DOI
- 10.1262/jrd.11-067H
- ISSN
- 0916-8818
1348-4400
- Abstract
- To predict the fertility of frozen-thawed bull spermatozoa, a sperm penetration assay (SPA) using zona-free hamster oocytes was optimized, and the assay results were compared with data from field fertility expressed as the non-return rate (NRR). To increase sperm penetration, the spermatozoa were pre-incubated and coincubated with oocytes in media containing various concentrations of heparin (0 to 50 mu g/ml). Coincubation with 10 mu g/ml heparin showed the highest sperm penetration (P<0.05); it is considered to be the optimized SPA method. Sperm fertility index values obtained from WSPA were significantly correlated with the historic average NRR of 46 bulls (P<0.01). To determine the normal range for SPA, we established the lower limits of the sperm fertility index and set the cut-off value at 2.55, at which point the NRR was more than 70 A,, using the receiver operating characteristic curve. The overall accuracy for the 46 bulls was 95.7% (44/46) for both the low and high NRR, with a sensitivity of 95.5% (21/22) and a specificity of 95.8%. This protocol would make it easier to discriminate bulls according to their sperm fertilizing ability.
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