Synthesis of Bioactive Microcapsules Using a Microfluidic Deviceopen access
- Authors
- Kim, Byeong Il; Jeong, Soon Woo; Lee, Kyoung G.; Park, Tae Jung; Park, Jung Youn; Song, Jae Jun; Lee, Seok Jae; Lee, Chang-Soo
- Issue Date
- Aug-2012
- Publisher
- MDPI AG
- Keywords
- microcapsulation; NIPAM; hydrogel; microfluidic device; spore
- Citation
- SENSORS, v.12, no.8, pp 10136 - 10147
- Pages
- 12
- Journal Title
- SENSORS
- Volume
- 12
- Number
- 8
- Start Page
- 10136
- End Page
- 10147
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/15224
- DOI
- 10.3390/s120810136
- ISSN
- 1424-8220
1424-8220
- Abstract
- Bioactive microcapsules containing Bacillus thuringiensis (BT) spores were generated by a combination of a hydro gel, microfluidic device and chemical polymerization method. As a proof-of-principle, we used BT spores displaying enhanced green fluorescent protein (EGFP) on the spore surface to spatially direct the EGFP-presenting spores within microcapsules. BT spore-encapsulated microdroplets of uniform size and shape are prepared through a flow-focusing method in a microfluidic device and converted into microcapsules through hydrogel polymerization. The size of microdroplets can be controlled by changing both the dispersion and continuous flow rate. Poly(N-isoproplyacrylamide) (PNIPAM), known as a hydrogel material, was employed as a biocompatible material for the encapsulation of BT spores and long-term storage and outstanding stability. Due to these unique properties of PNIPAM, the nutrients from Luria-Bertani complex medium diffused into the microcapsules and the microencapsulated spores germinated into vegetative cells under adequate environmental conditions. These results suggest that there is no limitation of transferring low-molecular-weight-substrates through the PNIPAM structures, and the viability of microencapsulated spores was confirmed by the culture of vegetative cells after the germinations. This microfluidic-based microencapsulation methodology provides a unique way of synthesizing bioactive microcapsules in a one-step process. This microfluidic-based strategy would be potentially suitable to produce microcapsules of various microbial spores for on-site biosensor analysis.
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