Zavarzinia aquatilis sp. nov., isolated from a freshwater river

Abstract

A Gram-stain-negative, strictly aerobic, catalase-positive and oxidase-positive bacterium, designated strain HR-AS(T), was isolated from a water sample of the Han River located in the Republic of Korea. Cells were motile rods with a polar flagellum. Growth was observed at 5-35 degrees C (optimum of 25 degrees C) and pH 6-8 (optimum of pH 7) and in the presence of 0-2% (w/v) NaCl (optimum of 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AS(T) formed a tight phylogenic lineage with Zavarzinia compransoris LMG 5821(T). Strain HR-AS(T) was most closely related to Z. compransoris LMG 5821(T) with a 98.7% 16S rRNA gene sequence similarity and had very low similarities (below 91.0 %) to other type strains with validly published names. Average nucleotide identity and in silico DNA-DNA hybridization values between strain HR-AS' and Z. compransoris DSM 1231(T) were 80.4 and 23.1 %, respectively. Strain HR-AS(T) contained ubiquinone-10 as the major quinone and homospermidine and putrescine as the major polyamines. The major fatty acids were summed feature 8 (C-18:1 omega 6c and/or C-18:1 omega 7C), C-16:0 and C-18:1 2-OH. Strain HR-AS(T) contained diphosphatidylglycerol, an unidentified aminolipid and two unidentified phospholipids as major polar lipids. The DNA G+C content of strain HR-AS(T) was 67.2 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-AS(T) represents a novel species of the genus Zavarzinia, for which the name Zavarzinia aquatilis sp. nov. is proposed. The type strain is HR-AS(T) (=KACC 19412(T)=JCM 32263(T)).