Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells
- Authors
- Soe, Zar Chi; Kwon, Jun Bum; Thapa, Raj Kumar; Ou, Wenquan; Hanh Thuy Nguyen; Gautam, Milan; Oh, Kyung Taek; Choi, Han-Gon; Ku, Sae Kwang; Yong, Chul Soon; Kim, Jong Oh
- Issue Date
- Feb-2019
- Publisher
- MDPI
- Keywords
- doxorubicin; doxorubicin-resistant cancer; polymeric nanoparticles; transferrin
- Citation
- PHARMACEUTICS, v.11, no.2
- Journal Title
- PHARMACEUTICS
- Volume
- 11
- Number
- 2
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/18293
- DOI
- 10.3390/pharmaceutics11020063
- ISSN
- 1999-4923
1999-4923
- Abstract
- In this study, a transferrin (T-f)-conjugated polymeric nanoparticle was developed for the targeted delivery of the chemotherapeutic agent doxorubicin (Dox) in order to overcome multi-drug resistance in cancer treatment. Our objective was to improve Dox delivery for producing significant antitumor efficacy in Dox-resistant (R) breast cancer cell lines with minimum toxicity to healthy cells. The results of our experiments revealed that Dox was successfully loaded inside a transferrin (T-f)-conjugated polymeric nanoparticle composed of poloxamer 407 (F127) and 123 (P123) (Dox/F127&P123-T-f), which produced nanosized particles (similar to 90 nm) with a low polydispersity index (similar to 0.23). The accelerated and controlled release profiles of Dox from the nanoparticles were characterized in acidic and physiological pH and Dox/F127&P123-T-f enhanced Dox cytotoxicity in OVCAR-3, MDA-MB-231, and MDA-MB-231(R) cell lines through induction of cellular apoptosis. Moreover, Dox/F127&P123-T-f inhibited cell migration and altered the cell cycle patterns of different cancer cells. In vivo study in MDA-MB-231(R) tumor-bearing mice demonstrated enhanced delivery of nanoparticles to the tumor site when coated in a targeting moiety. Therefore, Dox/F127&P123-T-f has been tailored, using the principles of nanotherapeutics, to overcome drug-resistant chemotherapy.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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