Fabrication of electrochemical biosensor consisted of multi-functional DNA structure/porous au nanoparticle for avian influenza virus (H5N1) in chicken serum
- Authors
- Lee, Taek; Park, Sun Yong; Jang, Hongje; Kim, Ga-Hyeon; Lee, Yeonju; Park, Chulhwan; Mohammadniaei, Mohsen; Lee, Min-Ho; Min, Junhong
- Issue Date
- Jun-2019
- Publisher
- Elsevier Ltd
- Keywords
- Avian influenza virus detection; Hemagglutinin; Multi-functional DNA structure; DNA 3 way-junction; Electrochemical biosensor; Porous Au nanoparticle
- Citation
- Materials Science and Engineering C, v.99, pp 511 - 519
- Pages
- 9
- Journal Title
- Materials Science and Engineering C
- Volume
- 99
- Start Page
- 511
- End Page
- 519
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/18555
- DOI
- 10.1016/j.msec.2019.02.001
- ISSN
- 0928-4931
1873-0191
- Abstract
- Avian influenza virus (AIV) is one of the most harmful pathogens to living things due to its fast infection, various mutations, and dangerous symptoms. In this study, we fabricated a label-free AIV H5N1 biosensor composed of multi-functional DNA structure on a porous Au nanoparticles (pAuNPs) fabricated electrode using the electrochemical (EC) technique. As a multi-functional bioprobe, the DNA 3 way-junction (3WJ) was introduced. Each fragment of DNA 3WJ was rolled to recognition part (hemagglutinin (HA) protein detection aptamer), EC signal generation part (horseradish peroxidase (HRP)-mimicked DNAzyme), and immobilization part (Thiol group). Each fragment was assembled in order to form the DNA 3WJ for AI detection and the assembled structure was confirmed by native-tris boric acid magnesium polyacrylamide gel electrophoresis (TBM-PAGE). Moreover, in order to increase the electrochemical signal sensitivity, pAuNPs were synthesized. The property of pAuNPs was investigated by field emission scanning electron microscopy (FE-SEM), Transmission electron microscopy (TEM), Ultraviolet–visible (UV-VIS) spectroscopy and zeta potential analysis. The DNA 3WJ on pAuNPs-modified Au electrode was then prepared using the layer-by-layer (LbL) assembly method. FE-SEM and atomic force microscopy (AFM) were used to investigate the surface morphology. Cyclic voltammetry (CV) was carried out to confirm the HA protein binding to DNA 3WJ-modified electrode. Moreover, The HA protein can be detected 1 pM in HEPES solution and 1 pM in diluted-chicken serum, respectively. The present study showed label-free, simple fabrication, and easy-to-tailor detection elements for AIV. The present biosensor can be a powerful candidate for various virus detection platforms. © 2019 Elsevier B.V.
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