Anti-adipogenic effects of 1,25-dihydroxyvitamin D3 are mediated by the maintenance of the wingless-type MMTV integration site/beta-catenin pathwayopen access
- Authors
- Lee, Haeyong; Bae, Sungmin; Yoon, Yoosik
- Issue Date
- Nov-2012
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- 1,25-dihydroxyvitamin D3; adipogenesis; beta-catenin; vitamin D; wingless-type MMTV integration site
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.30, no.5, pp 1219 - 1224
- Pages
- 6
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
- Volume
- 30
- Number
- 5
- Start Page
- 1219
- End Page
- 1224
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20048
- DOI
- 10.3892/ijmm.2012.1101
- ISSN
- 1107-3756
1791-244X
- Abstract
- 1,25-di hydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D, was found to have anti-adipogenic activity, however, its mechanism of action has not been fully elucidated. In this study, 3T3-L1 preadipocytes were differentiated in the presence and absence of 1,25(OH)2D3, and the expression of the genes and proteins of the wingless-type MMTV integral ion site (WNT)/beta-catenin pathway were analyzed. While the expression of the members of the WNT/beta-catenin pathway were significantly downregulated during the adipogenesis of untreated 3T3-L1 cells, 1,25(OH)2D3 treatment was found to maintain the WNT/beta-catenin pathway. Among the members of the WNT/beta-catenin pathway, the levels of WNT10B and disheveled (DVL)2 as well as the phosphorylation of glycogen synthase kinase (GSK)3 beta were maintained by 1,25(OH)2D3 treatment. The levels of nuclear beta-catenin, which were downregulated during adipogenesis, were also maintained by 1,25(OH)2D3 treatment. The results of this study suggested that the anti-adipogenic effect of 1,25(OH)2D3 was mediated by the maintenance of the WNT/beta-catenin pathway, which was normally downregulated during adipogenesis.
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