Evaluation of monoclonal antibody-based immunohistochemistry for the detection of European and North American Porcine reproductive and respiratory syndrome virus and a comparison with in situ hybridization and reverse transcription polymerase chain reaction
- Authors
- Han, Kiwon; Seo, Hwi Won; Oh, Yeonsu; Kang, Ikjae; Park, Changhoon; Kang, Sang Hoon; Kim, Sung-Hoon; Lee, Bog-Hieu; Kwon, Byungjoon; Chae, Chanhee
- Issue Date
- Jul-2012
- Publisher
- SAGE PUBLICATIONS INC
- Keywords
- Immunohistochemistry; in situ hybridization; pigs; Porcine reproductive and respiratory syndrome virus
- Citation
- JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, v.24, no.4, pp 719 - 724
- Pages
- 6
- Journal Title
- JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
- Volume
- 24
- Number
- 4
- Start Page
- 719
- End Page
- 724
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20225
- DOI
- 10.1177/1040638712446507
- ISSN
- 1040-6387
1943-4936
- Abstract
- The objective of the present study was to compare the ability of 2 monoclonal antibodies (mAbs; SDOW17 and SR30) to detect types 1 and 2 Porcine reproductive and respiratory syndrome virus (PRRSV) in formalin-fixed, paraffin-embedded (FFPE) lung tissues by immunohistochemistry (IHC) and to compare the immunohistochemical results with in situ hybridization (ISH) and reverse transcription nested polymerase chain reaction (RT-nPCR) detection techniques. Lungs from 30 experimentally infected pigs (15 pigs with each genotype of PRRSV) and 20 naturally infected pigs (10 pigs with each genotype of PRRSV) with types 1 and 2 PRRSV, respectively, were used for the IHC, ISH, and RT-nPCR analyses. The SR30 mAb-based IHC detected significantly more type 1 PRRSV-positive cells in the accessory and caudal lobes from the experimentally infected pigs at 7 (P = 0.025) and 14 (P = 0.018) days postinoculation, respectively, compared to the SDOW17 mAb-based IHC. The results demonstrated that SR30 mAb-based IHC is useful for detecting both types 1 and 2 PRRSV antigen in FFPE lung tissues.
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Collections - College of Biotechnology & Natural Resource > School of Food Science and Technology > 1. Journal Articles
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