Application of a Reassortant Cucumber mosaic virus Vector for Gene Silencing in Tomato and Chili Pepper Plants
- Authors
- Hong, Jin Sung; Rhee, Sun-Ju; Kim, Eun-Ji; Kim, Tae-Sung; Ryu, Ki Hyun; Masuta, Chikara; Lee, Gung Pyo
- Issue Date
- Mar-2012
- Publisher
- KOREAN SOC PLANT PATHOLOGY
- Keywords
- Cucumber mosaic virus vector; endogenous gene; phytoene desaturase; RNA silencing
- Citation
- PLANT PATHOLOGY JOURNAL, v.28, no.1, pp 81 - 86
- Pages
- 6
- Journal Title
- PLANT PATHOLOGY JOURNAL
- Volume
- 28
- Number
- 1
- Start Page
- 81
- End Page
- 86
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20465
- DOI
- 10.5423/PPJ.NT.11.2011.0220
- ISSN
- 1598-2254
2093-9280
- Abstract
- We developed a reassortant RNA virus vector derived from Cucumber mosaic virus (CMV), which has advantages of very wide host range and can efficiently induce gene silencing in a few model plants. Certain CMV isolates, however, show limited host ranges presumably because they naturally co-evolved with their own hosts. We used a reassortant comprised of two strains of CMV, V-CMV and Gn-CMV, to broaden the host range and to develop a virus vector for virus-induced gene silencing (VIGS). Gn-CMV could infect chili pepper and tomato more efficiently than V-CMV. Gn-CMV RNA1, 3 and Y-CMV RNA2-A1 vector were newly reconstructed, and the transcript mixture of RNA I and 3 genomes of Gn-CMV and RNA2 genome of Y-CMV RNA2 containing portions of the endogenous phytoene desaturase (PDS) gene (CMV2A1::PDSs) was inoculated onto chili pepper (cv. Chung-yang), tomato (cvs. Bloody butcher, Tigerella, Silvery fir tree, and Czech bush) and Nicotianu benthamiana. All the tested plants infected by the reassortant CMV vector showed typical photo-bleaching phenotypes and reduced expression levels of PDS mRNA. These results suggest that the reassortant CMV vector would be a useful tool for the rapid induction of the RNA silencing of endogenous genes in chili pepper and tomato plants.
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