Detecting Hepatitis E Virus with a Reverse Transcription Polymerase Chain Reaction Enzyme-Linked Immunosorbent Assay
- Authors
- Seo, Dong Joo; Tahk, Hongmin; Lee, Kang Bum; Lee, Min Hwa; Son, Na Ry; Seo, Sheungwoo; Cheon, Doo-Sung; Lee, Bog-Hieu; Choi, Changsun
- Issue Date
- Mar-2012
- Publisher
- SPRINGER
- Keywords
- Hepatitis E virus (HEV); Reverse transcription polymerase chain reaction enzyme-linked immunosorbent assay (RT-PCR-ELISA); Detection
- Citation
- FOOD AND ENVIRONMENTAL VIROLOGY, v.4, no.1, pp 14 - 20
- Pages
- 7
- Journal Title
- FOOD AND ENVIRONMENTAL VIROLOGY
- Volume
- 4
- Number
- 1
- Start Page
- 14
- End Page
- 20
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20497
- DOI
- 10.1007/s12560-011-9073-6
- ISSN
- 1867-0334
1867-0342
- Abstract
- This study aimed to develop a specific and sensitive reverse transcription polymerase chain reaction enzyme-linked immunosorbent assay (RT-PCR-ELISA) for detecting hepatitis E virus (HEV). Eight sets of primers and biotinylated probes designed in the ORF2-ORF3 overlapping region of HEV were tested for sensitivity. The ability of nested reverse transcription polymerase chain reaction (RT-PCR) and RT-PCR-ELISA to detect HEV was compared. RT-PCR-ELISA was 10-100 times more sensitive than nested RT-PCR and could detect 0.01 ng/mu l HEV in swine stool samples. In terms of specificity, RT-PCR-ELISA did not falsely detect HEV when other viruses such as hepatitis A virus, rotavirus, norovirus genotype I, norovirus genotype II, and Feline calicivirus were present. Therefore, RT-PCR-ELISA appears to be a sensitive and specific method for detecting HEV.
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Collections - College of Biotechnology & Natural Resource > School of Food Science and Technology > 1. Journal Articles
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