WNT/beta-catenin pathway mediates the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum
- Authors
- Lee, Haeyong; Bae, Sungmin; Kim, Yeong Shik; Yoon, Yoosik
- Issue Date
- Sep-2011
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Adipogenesis; 3T3-L1; siRNA; Therapeutic compounds; Adipogenic molecular biomarkers
- Citation
- LIFE SCIENCES, v.89, no.11-12, pp 388 - 394
- Pages
- 7
- Journal Title
- LIFE SCIENCES
- Volume
- 89
- Number
- 11-12
- Start Page
- 388
- End Page
- 394
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21265
- DOI
- 10.1016/j.lfs.2011.07.006
- ISSN
- 0024-3205
1879-0631
- Abstract
- Aims: This study was conducted to suggest the role of WNT/beta-catenin pathway in the anti-adipogenic effect of platycodin D, a natural compound found in Platycodon grandiflorum. Main methods: Gene knockdown experiments using small interfering RNA (siRNA) transfection were conducted to elucidate crucial role of beta-catenin in the anti-adipogenic effects of platycodin D. Real-Time PCR and Western blot were used to analyze the expression levels of mRNAs and proteins in the WNT/beta-catenin pathway. Key findings: During the adipocyte differentiation of 313-L1 cells, members of the WNT/beta-catenin pathway were normally down-regulated, whereas platycodin D significantly reinstated the WNT/beta-catenin pathway. The mRNA and protein expressions of disheveled (DVL) 2, which stabilize beta-catenin, were increased by platycodin D treatment, but the protein level of AXIN, which induces the degradation of beta-catenin, was decreased in platycodin D-treated cells. The nuclear level of beta-catenin was normally down-regulated during adipogenesis, but platycodin D treatment led to the accumulation of beta-catenin in the nucleus which resulted in the up-regulation of its target genes, cyclin D (CCND) 1 and peroxisome proliferator-activated receptor gamma (PPAR)gamma. The anti-adipogenic effects of platycodin D were significantly attenuated in beta-catenin siRNA-transfected cells compared with those of control siRNA-transfected cells. beta-catenin siRNA transfection significantly recovered the levels of PPAR gamma. CCAAT/enhancer binding protein (C/EBP)alpha and fatty acid binding protein (FABP)4 as well as intracellular lipid droplet formation, all of which were reduced by platycodin D treatment. Significance: WNT/beta-catenin pathway can be used as a therapeutic target of natural compounds for the regulation of adipogenesis. (C) 2011 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21265)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.