Ultrasonic-assisted Micellar Extraction and Cloud-point Pre-concentration of Major Saikosaponins in Radix Bupleuri using High Performance Liquid Chromatography with Evaporative Light Scattering Detection
- Authors
- Suh, Joon Hyuk; Yang, Dong-Hyug; Han, Sang Beom
- Issue Date
- 20-Aug-2011
- Publisher
- KOREAN CHEMICAL SOC
- Keywords
- Saikosaponin; Cloud-point extraction; Genapol X-080; Radix Bupleuri; HPLC-ELSD
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.32, no.8, pp 2637 - 2642
- Pages
- 6
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 32
- Number
- 8
- Start Page
- 2637
- End Page
- 2642
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21334
- DOI
- 10.5012/bkcs.2011.32.8.2637
- ISSN
- 0253-2964
1229-5949
- Abstract
- A new ultrasonic-assisted micellar extraction and cloud-point pre-concentration method was developed for the determination of major saikosaponins, namely saikosaponins -A, -C and -D, in Radix Bupleuri by high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD). The non-ionic surfactant Genapol X-080 (oligoethylene glycol monoalkyl ether) was chosen as the extraction additive and parameters affecting the extraction efficiency were optimized. The highest yield was obtained with 10% (w/v) Genapol X-080, a liquid/solid ratio of 200:1 (mL/g) and ultrasonic-assisted extraction for 40 min. In addition, the optimum cloud-point pre-concentration was reached with 10% sodium sulfate and equilibration at 60 degrees C for 30 min. Separation was achieved on an Ascentis Express C18 column (100 x 4.6 mm i.d., 2.7 mu m) using a binary mobile phase composed of 0.1% acetic acid and acetonitrile. Saikosaponins were detected by ELSD, which was operated at a 50 degrees C drift tube temperature and 3.0 bar nebulizer gas (N(2)) pressure. The water-based solvent modified with Genapol X-080 showed better extraction efficiency compared to that of the conventional solvent methanol. Recovery of saikosaponins ranged from 93.1 to 101.9%. An environmentally-friendly extraction method was successfully applied to extract and enrich major saikosaponins in Radix Bupleuri.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21334)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.