The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis
- Authors
- Moon, Jeong Yong; Mosaddik, Ashik; Kim, Hana; Cho, Moonjae; Choi, Hyung-Kyoon; Kim, Young Suk; Cho, Somi Kim
- Issue Date
- Mar-2011
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Apoptosis; Guava; Caspase-3; High performance liquid chromatography coupled with photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS); Human gastric carcinoma cell; Phytochemical; SNU-16
- Citation
- FOOD CHEMISTRY, v.125, no.2, pp 369 - 375
- Pages
- 7
- Journal Title
- FOOD CHEMISTRY
- Volume
- 125
- Number
- 2
- Start Page
- 369
- End Page
- 375
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21671
- DOI
- 10.1016/j.foodchem.2010.09.007
- ISSN
- 0308-8146
- Abstract
- The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 mu g/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 mu g/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3', 4', 5' trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer. (C) 2010 Elsevier Ltd All rights reserved.
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