Expression of human β-defensin-2 in the prostateopen access
- Authors
- Kim, Hae Jong; Jung, Ja Rang; Kim, Hea Jin; Lee, Shin Young; Chang, In Ho; Lee, Tae Jin; Kim, Wonyong; Myung, Soon Chul
- Issue Date
- Jan-2011
- Publisher
- WILEY
- Keywords
- prostate; human beta-defensin-2; lipopolysaccharide; NF-kappa B activation
- Citation
- BJU INTERNATIONAL, v.107, no.1, pp 144 - 149
- Pages
- 6
- Journal Title
- BJU INTERNATIONAL
- Volume
- 107
- Number
- 1
- Start Page
- 144
- End Page
- 149
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21841
- DOI
- 10.1111/j.1464-410X.2010.09469.x
- ISSN
- 1464-4096
1464-410X
- Abstract
- We found the expression of human beta-defensin-2 (HBD-2) in the prostate for the first time and LPS, a gram negative bacterial component, upregulated HBD-2 in prostate epithelial cells. We are looking for other antimicrobial peptides expressed in the prostate besides human beta-defensin-2. Also, we are studying the relationship between antimicrobial peptides and the development or progression of prostate diseases. OBJECTIVE To investigate the expression and regulation of human beta-defensin-2 (HBD-2) in the prostate. PATIENTS AND METHODS Normal human prostate epithelial cell line (RWPE-1), human prostate cancer cell lines (DU-145, PC-3), and paraffin-embedded prostate tissue from patients with benign prostatic hyperplasia (BPH) were analysed by RT-PCR and immunohistochemical staining. HBD-2 expression was also analysed by RT-PCR and ELISA in RWPE-1 cells treated with lipopolysaccharide (LPS). Nuclear factor-kappa B (NF-kappa B) activation was assessed by I kappa B alpha immunoblotting and electrophoretic mobility shift assay (EMSA). RESULTS BPH tissue and all of the tested prostate cell lines other than PC-3 constitutively express HBD-2 mRNA. HBD-2 protein was strongly detected in prostate gland tissue surrounded by inflammatory cells including macrophages. Exposure to LPS induced HBD-2 upregulation and NF-kappa B activation, as assessed by I kappa B alpha phosphorylation and degradation in RWPE-1 cells. Bay11-7082, an NF-kappa B inhibitor prevented LPS-induced HBD-2 production in RWPE-1 cells. CONCLUSIONS Prostate epithelial cells may constitutively express HBD-2, and its expression was upregulated by LPS. Our data indicate that HBD-2 may be an important immunomodulatory factor in prostate function. Expression of HBD-2 in normal prostates and the potential role of HBD-2 in prostatitis and BPH should be addressed in the future.
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