Escherichia coli 16S rRNA 상의 770 위치에 염기치환을 가진 변이체 리보솜의 단백질 합성 능력을 회복시키는 이차복귀돌연변이체의 발췌Functional Analysis and Selection of Second-site Revertant of Escherichia coli 16S rRNA of C770G
- Authors
- 하혜정; 류상미; 이강석; 전체옥
- Issue Date
- Mar-2011
- Publisher
- 한국미생물·생명공학회
- Keywords
- B2c; Error-prone PCR; Ribosome; rRNA
- Citation
- Korean Journal of Microbiology and Biotechnology, v.39, no.1, pp 93 - 96
- Pages
- 4
- Journal Title
- Korean Journal of Microbiology and Biotechnology
- Volume
- 39
- Number
- 1
- Start Page
- 93
- End Page
- 96
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21890
- ISSN
- 1598-642X
2234-1305
- Abstract
- It has been shown that a nucleotide substitution at position 770 in Escherichia coli 16S rRNA, which is implicated in forming the evolutionary conserved B2c intersubunit bridge, has a detrimental effect on ribosome function. In order to isolate second-site revertants that complement ribosomes containing C770G, we performed a random mutagenesis of the 16S rRNA gene and selected clones that could produce more CAT protein translated by specialized ribosome. One of the clones contained two nucleotide substitutions at positions 569 and 904 (C569G and U904C) and these mutations partially complemented the loss of protein-synthesis ability caused by C770G. Further studies using the isolated revertant will provide information about which part of 16S rRNA is interacting with C770 and the consequence of the structure formed by these interactions in the process of protein synthesis.
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