Delivery of shRNA using gold nanoparticle-DNA oligonucleotide conjugates as a universal carrier
- Authors
- Ryou, Sang-Mi; Kim, Sudeok; Jang, Hyun Hye; Kim, Jae-Hong; Yeom, Ji-Hyun; Eom, Min Sik; Bae, Jeehyeon; Han, Min Su; Lee, Kangseok
- Issue Date
- Jul-2010
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Gold nanoparticle; Gene delivery system; Gene knockdown; shRNA
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.398, no.3, pp 542 - 546
- Pages
- 5
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 398
- Number
- 3
- Start Page
- 542
- End Page
- 546
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22320
- DOI
- 10.1016/j.bbrc.2010.06.115
- ISSN
- 0006-291X
1090-2104
- Abstract
- The efficient delivery of nucleic acids into mammalian cells is a central aspect of research involving cell biology and medical applications, including the clinical treatment of genetic disorders. We report an efficient small hairpin RNA (shRNA) delivery system that utilizes a single species of gold nanoparticle-DNA oligonucleotide conjugate (AuNP-DNA oligo) as a universal carrier. In vitro synthesized shRNA that is specific to the p53 gene was efficiently delivered into HEK293 and HeLa human cell lines using an AuNP-DNA oligo. The delivery resulted in an 80-90% knockdown of p53 expression. The same AuNP-DNA oligo was also efficient for the delivery of another shRNA, which is specific to the Mcl-1 gene, as well as the repression of MCL-1 expression. The knockdown efficiency of shRNA that was delivered using an AuNP-DNA oligo was comparable with that of a liposome-based shRNA delivery method. Our results offer an alternate delivery system for shRNA that can be used on any gene of interest. (C) 2010 Elsevier Inc. All rights reserved.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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