Determination of hyperin in the fruits of Acanthopanax species by high performance liquid chromatography

Abstract

The content of hyperin in Acanthopanax species was determined by high performance liquid chromatography (HPLC). Hyperin was quantified by a reverse-phase column with elution program [initially gradient solvent (acetonitrile: water = 85: 15 to 80: 20 for 20 min), then isocratic solvent (acetonitrile: water=80: 20 for 20 min), and finally gradient solvent (acetonitrile: water = 80: 20 to 65: 35 for 20 min)]. UV detection was conducted at 210 nm. The content of hyperin in the fruits of Acanthopanax was measured in the species A. chiisanensis (2.04 mg/g), A. sessiliflorus (1.13 mg/g), A. divaricatus (0.98 mg/g), A. koreanum (0.75 mg/g) and A. senticosus (0.05 mg/g). The content of hyperin in A. chiisanensis was higher than that of other Acanthopanax species.