Induction of IL-8 expression by bacterial flagellin is mediated through lipid raft formation and intracellular TLR5 activation in A549 cells
- Authors
- Im, Jintaek; Jeon, Jun Ho; Cho, Min Kyung; Woo, Sang Su; Kang, Seok-Seong; Yun ,Cheol-Heui; Lee, Kangseok; Chung, Dae Kyun; Han, Seung Hyun
- Issue Date
- Dec-2009
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Flagellin; IL-8; Toll-like receptor 5; Lipid rafts; A549
- Citation
- MOLECULAR IMMUNOLOGY, v.47, no.2-3, pp 614 - 622
- Pages
- 9
- Journal Title
- MOLECULAR IMMUNOLOGY
- Volume
- 47
- Number
- 2-3
- Start Page
- 614
- End Page
- 622
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22859
- DOI
- 10.1016/j.molimm.2009.09.004
- ISSN
- 0161-5890
- Abstract
- We investigated the mechanism for the induction of a chemokine, IL-8, by bacterial flagellins in the human alveolar type 11 epithelial cell line, A549. Bacterial flagellin induced expression of IL-8 mRNA and protein in dose- and time-dependent manners. IL-8 expression was inhibited by nystatin (a lipid rafts inhibitor) but not by chlorpromazine (a clathrin-coated pits inhibitor). Interestingly, Toll-like receptor 5 (TLR5) recognizing flagellins was found in the intracellular compartment of A549 but rarely on the cell surface. Flagellin-induced IL-8 expression appears to be mediated through TLR5 as determined by in vitro transient transfection experiment in HEK-293 cells expressing TLR5 using a reporter gene construct containing IL-8 promoter. IL-8 expression was attenuated by inhibitors for protein kinase C (PKC) and mitogen-activated protein (MAP) kinases. Furthermore, NF-kappa B and NF-IL6 transcription factors played an important role in the flagellin-induced IL-8 gene expression in A549 cells. Collectively, these results suggest that flagellin-induced IL-8 expression requires formation of lipid rafts, intracellular TLR activation. and subsequent activation of PKC and MAP kinases leading to the activation of the transcription factors NF-kappa B and NF-IL6 in human alveolar type II epithelial cells. (C) 2009 Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22859)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.