Paraburkholderia aromaticivorans sp nov., an aromatic hydrocarbon-degrading bacterium, isolated from gasoline-contaminated soil

Abstract

A Gram-stain-negative, facultatively aerobic, aromatic hydrocarbon-degrading bacterium, designated strain BN5(T), was isolated from gasoline-contaminated soil. Cells were motile and slightly curved rods with a single flagellum showing catalase and oxidase activities. Growth was observed at 20-37 degrees C (optimum, 25-30 degrees C), pH 3-7 (optimum, pH 5-6) and 0-2 % NaCl (optimum, 0 %).Ubiquinone-8 was the predominant respiratory quinone. The major fatty acids were C-16:0, cyclo-C-19:0 omega 8c and summed feature 8 (comprising C-18:1 omega 7c and/or C-18:1 omega 6c). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phosphoamino lipid, three unidentified amino lipids and eight unidentified lipids were the identified polar lipids. The DNA G+C content was 62.93 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BN5(T) formed a phylogenic lineage with members of the genus Paraburkholderia and showed the highest 16S rRNA gene sequence similarities to Paraburkholderia phytofirmans PsJN(T) (99.4 %), Paraburkholderia dipogonis DL7(T) (98.8 %) and Paraburkholderia insulsa PNG-April(T) (98.8 %). The average nucleotide identity and in silico DNA-DNA hybridization (DDH) values between strain BN5(T) and P. phytofirmans PsJN(T) were 88.5 and 36.5 %, respectively. The DDH values for strain BN5(T) with P. dipogonis LMG 28415(T) and P. insulsa DSM 28142(T) were 41.0 +/- 4.9 % (reciprocal, 33.0 +/- 4.3 %) and 47.1 +/- 6.6 % (reciprocal, 51.7 +/- 5.4 %), respectively. Based on its physiological, chemotaxonomic and phylogenetic features, we conclude that strain BN5(T) is a novel species of the genus Paraburkholderia, for which the name Paraburkholderia aromaticivorans sp. nov. is proposed. The type strain is BN5(T) (=KACC 19419(T)=JCM 32303(T)).