Bacterial community composition and chitinase gene diversity of vermicompost with antifungal activity
- Authors
- Yasir, Muhammad; Aslam, Zubair; Kim, Seon Won; Lee, Seon-Woo; Jeon, Che Ok; Chung, Young Ryun
- Issue Date
- Oct-2009
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Bacterial community; Chitinase; Plant pathogenic fungi; Paper sludge; Vermicomposting
- Citation
- BIORESOURCE TECHNOLOGY, v.100, no.19, pp 4396 - 4403
- Pages
- 8
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 100
- Number
- 19
- Start Page
- 4396
- End Page
- 4403
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22999
- DOI
- 10.1016/j.biortech.2009.04.015
- ISSN
- 0960-8524
1873-2976
- Abstract
- Bacterial communities and chitinase gene diversity of vermicompost (VC) were investigated to clarify the influence of earthworms on the inhibition of plant pathogenic fungi in VC. The spore germination of Fusarium moniliforme was reduced in VC aqueous extracts prepared from paper sludge and dairy sludge (fresh sludge, FS). The bacterial communities were examined by culture-dependent and -independent analyses. Unique clones selected from 16S rRNA libraries of FS and VC on the basis of restriction fragment length polymorphism (RFLP) fell into the major lineages of the domain bacteria Proteobacteria, Bacteroidetes, Verrucomicrobia, Actinobacteria and Firmicutes. Among culture isolates, Actinobacteria dominated in VC, while almost equal numbers of Actinobacteria and Proteobacteria were present in FS. Analysis of chitinolytic isolates and chitinase gene diversity revealed that chitinolytic bacterial communities were enriched in VC. Populations of bacteria that inhibited plant fungal pathogens were higher in VC than in FS and particularly chitinolytic isolates were most active against the target fungi. (C) 2009 Elsevier Ltd. All rights reserved.
- Files in This Item
-
- Appears in
Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.